PhanerochaeteV1, Main, Exploration, bibRecord, 000593

[Induction and measurement of cytochrome P450 in white rot fungi].

Identifieur interne : 000593 ( Main/Exploration ); précédent : 000592; suivant : 000594

[Induction and measurement of cytochrome P450 in white rot fungi].

Auteurs : Da-Liang Ning [République populaire de Chine] ; Hui Wang ; Dong Li

Source :

Huan jing ke xue= Huanjing kexue [ 0250-3301 ] ; 2009.

RBID : pubmed:19799321

Descripteurs français

KwdFr :
- Analyse spectrale (méthodes), Cytochrome P-450 enzyme system (analyse), Cytochrome P-450 enzyme system (métabolisme), Dithionite (pharmacologie), Hexanes (pharmacologie), Induction enzymatique (effets des médicaments et des substances chimiques), Phanerochaete (enzymologie).
MESH :
- analyse : Cytochrome P-450 enzyme system.
- effets des médicaments et des substances chimiques : Induction enzymatique.
- enzymologie : Phanerochaete.
- métabolisme : Cytochrome P-450 enzyme system.
- méthodes : Analyse spectrale.
- pharmacologie : Dithionite, Hexanes.

English descriptors

KwdEn :
- Cytochrome P-450 Enzyme System (analysis), Cytochrome P-450 Enzyme System (metabolism), Dithionite (pharmacology), Enzyme Induction (drug effects), Hexanes (pharmacology), Phanerochaete (enzymology), Spectrum Analysis (methods).
MESH :
- chemical , analysis : Cytochrome P-450 Enzyme System.
- chemical , metabolism : Cytochrome P-450 Enzyme System.
- chemical , pharmacology : Dithionite, Hexanes.
- drug effects : Enzyme Induction.
- enzymology : Phanerochaete.
- methods : Spectrum Analysis.

Abstract

The induction and measurement of cytochrome P450 in white rot fungus Phanerochaete chrysosporium were studied in this work. The spectrophotometric results demonstrated that n-hexane was able to induce the fungal P450 to high level, which facilitated isolation and measurement of microsomal P450. The highest concentration of microsomal P450 could reach 140-160 pmol/mg after 6-h-induction by addition of 2 microL/mL hexane each hour, and the concentration of hexane and incubation time had significant effect on the induction of P450s. After effective induction, the method for isolation and measurement of microsomal P450 with CO difference spectrum was studied and the optimized method was obtained as followed. High-speed disperser and glass homogenizer were used to disrupt cells, which obtained higher amount of microsomal P450 than those from cells disrupted by glass homogenizer, ultrasonicator and bead-beater respectively. To record CO difference spectrum,the sample was bubbled with CO for 40 s at a rate of 3 mL/min (300 microL sample), and the reference cuvette was bubbled with N2 to the same extent. Then, the reducer sodium dithionite was added to a concentration 0.4 mol/L.

PubMed: 19799321

Affiliations:

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Le document en format XML

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<author><name sortKey="Ning, Da Liang" sort="Ning, Da Liang" uniqKey="Ning D" first="Da-Liang" last="Ning">Da-Liang Ning</name>
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<term>Enzyme Induction (drug effects)</term>
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<term>Phanerochaete (enzymology)</term>
<term>Spectrum Analysis (methods)</term>
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<term>Cytochrome P-450 enzyme system (analyse)</term>
<term>Cytochrome P-450 enzyme system (métabolisme)</term>
<term>Dithionite (pharmacologie)</term>
<term>Hexanes (pharmacologie)</term>
<term>Induction enzymatique (effets des médicaments et des substances chimiques)</term>
<term>Phanerochaete (enzymologie)</term>
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<keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>Cytochrome P-450 Enzyme System</term>
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<front><div type="abstract" xml:lang="en">The induction and measurement of cytochrome P450 in white rot fungus Phanerochaete chrysosporium were studied in this work. The spectrophotometric results demonstrated that n-hexane was able to induce the fungal P450 to high level, which facilitated isolation and measurement of microsomal P450. The highest concentration of microsomal P450 could reach 140-160 pmol/mg after 6-h-induction by addition of 2 microL/mL hexane each hour, and the concentration of hexane and incubation time had significant effect on the induction of P450s. After effective induction, the method for isolation and measurement of microsomal P450 with CO difference spectrum was studied and the optimized method was obtained as followed. High-speed disperser and glass homogenizer were used to disrupt cells, which obtained higher amount of microsomal P450 than those from cells disrupted by glass homogenizer, ultrasonicator and bead-beater respectively. To record CO difference spectrum,the sample was bubbled with CO for 40 s at a rate of 3 mL/min (300 microL sample), and the reference cuvette was bubbled with N2 to the same extent. Then, the reducer sodium dithionite was added to a concentration 0.4 mol/L.</div>
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<Month>09</Month>
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<Day>18</Day>
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<Abstract><AbstractText>The induction and measurement of cytochrome P450 in white rot fungus Phanerochaete chrysosporium were studied in this work. The spectrophotometric results demonstrated that n-hexane was able to induce the fungal P450 to high level, which facilitated isolation and measurement of microsomal P450. The highest concentration of microsomal P450 could reach 140-160 pmol/mg after 6-h-induction by addition of 2 microL/mL hexane each hour, and the concentration of hexane and incubation time had significant effect on the induction of P450s. After effective induction, the method for isolation and measurement of microsomal P450 with CO difference spectrum was studied and the optimized method was obtained as followed. High-speed disperser and glass homogenizer were used to disrupt cells, which obtained higher amount of microsomal P450 than those from cells disrupted by glass homogenizer, ultrasonicator and bead-beater respectively. To record CO difference spectrum,the sample was bubbled with CO for 40 s at a rate of 3 mL/min (300 microL sample), and the reference cuvette was bubbled with N2 to the same extent. Then, the reducer sodium dithionite was added to a concentration 0.4 mol/L.</AbstractText>
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[Induction and measurement of cytochrome P450 in white rot fungi].

[Induction and measurement of cytochrome P450 in white rot fungi].

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri

Pour générer des pages wiki